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Anti-Periostin/OSF-2 Antibody (2I76) is an antibody targeting Periostin/OSF-2. Anti-Periostin/OSF-2 Antibody (2I76) can be used in ELISA, IHC, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, IHC, FCM Reactivity: Human |
Alias | PN, periostin, osteoblast specific factor, periostin, PDLPOSTN, OSF-2, OSF2 |
Ig Type | Rabbit IgG |
Clone | 2I76 |
Reactivity | Human |
Verified Activity | 1. IHC image of TMAH-00995 diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.31% DAB. 2. Overlay Peak curve showing 293T cells stained with TMAH-00995 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, IHC, FCM |
Recommended Dose | IHC:1:50-1:200; FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Golgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Induces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human POSTN |
Antigen Species | Human |
Gene ID | 10631 |
Uniprot ID | |
Biology Area | Cancer, Cardiovascular, Tags & Cell Markers, Signal transduction |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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