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Anti-Periostin/OSF-2 Antibody (2I76)

😃Good
Catalog No. TMAH-00995
Alias PN, periostin, osteoblast specific factor, periostin, PDLPOSTN, OSF-2, OSF2

Anti-Periostin/OSF-2 Antibody (2I76) is an antibody targeting Periostin/OSF-2. Anti-Periostin/OSF-2 Antibody (2I76) can be used in ELISA, IHC, FCM.

Anti-Periostin/OSF-2 Antibody (2I76)

Anti-Periostin/OSF-2 Antibody (2I76)

😃Good
Catalog No. TMAH-00995Alias PN, periostin, osteoblast specific factor, periostin, PDLPOSTN, OSF-2, OSF2
Anti-Periostin/OSF-2 Antibody (2I76) is an antibody targeting Periostin/OSF-2. Anti-Periostin/OSF-2 Antibody (2I76) can be used in ELISA, IHC, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$347 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, IHC, FCM

Reactivity: Human
AliasPN, periostin, osteoblast specific factor, periostin, PDLPOSTN, OSF-2, OSF2
Ig Type
Rabbit IgG
Clone
2I76
Reactivity
Human
Verified Activity
1. IHC image of TMAH-00995 diluted at 1:50 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.31% DAB.
2. Overlay Peak curve showing 293T cells stained with TMAH-00995 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, IHC, FCM
Recommended Dose
IHC:1:50-1:200; FCM:1:50-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationGolgi apparatus. Secreted. Secreted, extracellular space, extracellular matrix.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundInduces cell attachment and spreading and plays a role in cell adhesion. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human POSTN
Antigen Species
Human
Gene ID
10631
Uniprot ID
Biology Area
Cancer, Cardiovascular, Tags & Cell Markers, Signal transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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