Antibody Type: Recombinant Monoclonal

Application: ELISA, WB, IHC, FC

Reactivity: Human

mIgG2a

9M512

Human

1. Western Blot
-Positive WB detected in:K562 whole cell lysate (20µg)
-All lanes: GZMB antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size:27.7 kDa
-Observed band size:28 kDa
-Exposure time:300s
2. IHC image of TMAH-00527 diluted at 1:50 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
3. Overlay Peak curve showing THP-1 cells stained with TMAH-00527 (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was mouse IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

ELISA, WB, IHC, FC

WB:1:500-1:2000; IHC:1:50-1:200; FC:1:50-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human GZMB Protein

Human

3002

Cell biology, Immunology