SEMA3A Protein, Human, Recombinant (His)

Name: SEMA3A Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPK-02011
Rating: 4.5 (1 reviews)

(Synonyms: SemD, Semaphorin III, Semaphorin 3A, SEMAL, SEMAIII, SEMAD, SEMA3A, SEMA-1, SEMA1, Sema III, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, SEMA 1, Hsema-III, Hsema-I, HH-16, HH16, HH 16, coll-1, COLL-1, COLL1, COLL 1) Copy Product Info

Synonyms: SemD, Semaphorin III, Semaphorin 3A, SEMAL, SEMAIII, SEMAD, SEMA3A, SEMA-1, SEMA1, Sema III, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, SEMA 1, Hsema-III, Hsema-I, HH-16, HH16, HH 16, coll-1, COLL-1, COLL1, COLL 1

Catalog No. TMPK-02011 Copy Product Info

SEMA3A Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q14563.

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Pack Size	Price	USA Stock	Global Stock
5 µg	$84	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$196	7-10 days	7-10 days
50 µg	$365	7-10 days	7-10 days
100 µg	$577	7-10 days	7-10 days
200 µg	$918	7-10 days	7-10 days
500 µg	$1,700	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Immobilized Anti-SEMA3A Antibody at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30 μg/ml determined by ELISA.
Description	SEMA3A Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q14563.
Species	Human
Expression System	HEK293 Cells
Tag	N-His
Accession Number	Q14563
Construction	Asn21-Val771
Protein Purity	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin	< 1 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	SemD, Semaphorin III, Semaphorin 3A, SEMAL, SEMAIII, SEMAD, SEMA3A, SEMA-1, SEMA1, Sema III, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, SEMA 1, Hsema-III, Hsema-I, HH-16, HH16, HH 16, coll-1, COLL-1, COLL1, COLL 1
Research Background	The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain.

Chemical Properties

Molecular Weight

87.7 kDa (Predicted); 60-70 kDa (Due to furin cleavage site)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.