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SEMA3A Protein, Human, Recombinant (His)
(Synonyms: SemD, Semaphorin III, Semaphorin 3A, SEMAL, SEMAIII, SEMAD, SEMA3A, SEMA-1, SEMA1, Sema III, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, SEMA 1, Hsema-III, Hsema-I, HH-16, HH16, HH 16, coll-1, COLL-1, COLL1, COLL 1) Copy Product InfoSynonyms: SemD, Semaphorin III, Semaphorin 3A, SEMAL, SEMAIII, SEMAD, SEMA3A, SEMA-1, SEMA1, Sema III, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, SEMA 1, Hsema-III, Hsema-I, HH-16, HH16, HH 16, coll-1, COLL-1, COLL1, COLL 1
Catalog No. TMPK-02011 Copy Product Info
SEMA3A Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q14563.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Anti-SEMA3A Antibody at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human SEMA3A, His Tag with the EC50 of 1.30 μg/ml determined by ELISA. |
| Description | SEMA3A Protein, Human, Recombinant (His) is expressed in HEK293 Cells. The accession number is Q14563. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | N-His |
| Accession Number | Q14563 |
| Construction | Asn21-Val771 |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | SemD, Semaphorin III, Semaphorin 3A, SEMAL, SEMAIII, SEMAD, SEMA3A, SEMA-1, SEMA1, Sema III, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A, SEMA 1, Hsema-III, Hsema-I, HH-16, HH16, HH 16, coll-1, COLL-1, COLL1, COLL 1 |
| Research Background |
| Molecular Weight | 87.7 kDa (Predicted); 60-70 kDa (Due to furin cleavage site) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: SEMA3A Protein, Human, Recombinant (His) chemical structure | SEMA3A Protein, Human, Recombinant (His) molecular weight
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