Immobilized Human Her3, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 26.9 ng/ml determined by ELISA.
Immobilized Human Her3, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-HER3 Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA.

HER3/ERBB3 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is P21860-1.

Human

HEK293 Cells

p85-sErbB3,p45-sErbB3,p180-ErbB3,MDA-BF-1,LCCS2,HER3,erbB3-S,ErbB-3,erb-b2 receptor tyrosine kinase 3,EEBB3,c-erbB-3,c-erbB3

Ser20-Thr643

> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).