Shopping Cart
- Remove All
- Your shopping cart is currently empty
Anti-OCT4 Antibody (7S23) is a Mouse antibody targeting OCT4. Anti-OCT4 Antibody (7S23) can be used in ELISA, WB, IHC, IF, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, IHC, IF, FCM Reactivity: Human, Mouse, Rat |
Ig Type | IgG2b |
Clone | 7S23 |
Reactivity | Human, Mouse, Rat |
Verified Activity | 1. Western Blot -Positive WB detected in: HepG2 whole cell lysate -All lanes: OCT4 antibody at 1:500 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 39, 31 kDa -Observed band size: 45 kDa 2. Western Blot -Positive WB detected in: Mouse brain tissue, Rat brain tissue -All lanes: OCT4 antibody at 1:1000, 1:5000, 1:8000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 39, 31 kDa -Observed band size: 45 kDa 3. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 4. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 5. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 6. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 7. Immunofluorescence staining of A549 cells with TMAH-00842 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 8. Immunofluorescence staining of Ntera-2 cells with TMAH-00842 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 9. Overlay histogram showing A549 cells stained with TMAH-00842 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. 10. Overlay histogram showing Ntera-2 cells stained with TMAH-00842 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. |
Application | ELISA, WB, IHC, IF, FCM |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Cytoplasm. Nucleus. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Purity | >95% |
Research Background | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human POU domain, class 5, transcription factor 1 Protein (1-360AA) |
Antigen Species | Human |
Gene ID | 5460 |
Uniprot ID | |
Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.