Shopping Cart
  • Remove All
  • TargetMol
    Your shopping cart is currently empty

Anti-OCT4 Antibody (7S23)

😃Good
Catalog No. TMAH-00842

Anti-OCT4 Antibody (7S23) is a Mouse antibody targeting OCT4. Anti-OCT4 Antibody (7S23) can be used in ELISA, WB, IHC, IF, FCM.

Anti-OCT4 Antibody (7S23)

Anti-OCT4 Antibody (7S23)

😃Good
Catalog No. TMAH-00842
Anti-OCT4 Antibody (7S23) is a Mouse antibody targeting OCT4. Anti-OCT4 Antibody (7S23) can be used in ELISA, WB, IHC, IF, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$349 7-10 days
Bulk & Custom
Add to Cart
Questions
View More
Contact us for more batch information
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Introduction

Bioactivity
Description
Antibody Type: Mouse Monoclonal

Application: ELISA, WB, IHC, IF, FCM

Reactivity: Human, Mouse, Rat
Ig Type
IgG2b
Clone
7S23
Reactivity
Human, Mouse, Rat
Verified Activity
1. Western Blot
-Positive WB detected in: HepG2 whole cell lysate
-All lanes: OCT4 antibody at 1:500
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 39, 31 kDa
-Observed band size: 45 kDa
2. Western Blot
-Positive WB detected in: Mouse brain tissue, Rat brain tissue
-All lanes: OCT4 antibody at 1:1000, 1:5000, 1:8000
-Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution
-Predicted band size: 39, 31 kDa
-Observed band size: 45 kDa
3. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
4. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
5. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
6. IHC image of TMAH-00842 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
7. Immunofluorescence staining of A549 cells with TMAH-00842 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
8. Immunofluorescence staining of Ntera-2 cells with TMAH-00842 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
9. Overlay histogram showing A549 cells stained with TMAH-00842 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
10. Overlay histogram showing Ntera-2 cells stained with TMAH-00842 (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
Application
ELISA, WB, IHC, IF, FCM
Antibody Type
Monoclonal
Host SpeciesMouse
Subcellular LocalizationCytoplasm. Nucleus.
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Purity>95%
Research BackgroundTranscription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human POU domain, class 5, transcription factor 1 Protein (1-360AA)
Antigen Species
Human
Gene ID
5460
Uniprot ID
Biology Area
Epigenetics and Nuclear Signaling
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

Sci Citations

Calculator

  • Molarity Calculator
  • Dilution Calculator
  • Reconstitution Calculator
  • Molecular Weight Calculator

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: buy Anti-OCT4 Antibody (7S23) | purchase Anti-OCT4 Antibody (7S23) | Anti-OCT4 Antibody (7S23) cost | order Anti-OCT4 Antibody (7S23)