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Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) (Synonyms: ubiquitin-like modifier activating enzyme 1...)
Catalog No. TMAY-02188 Copy Product Info
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Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) is a Mouse antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) can be used in ELISA,IP.
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888)
Copy Product Info🥰Excellent
Catalog No. TMAY-02188
Synonyms ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) is a Mouse antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) can be used in ELISA,IP.
Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody
(1K888)
(1K888)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 100 μL | $199 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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| Description | Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) is a Mouse antibody targeting Ubiquitin Activating Enzyme E1/UBA1. Anti-Ubiquitin Activating Enzyme E1/UBA1 Antibody (1K888) can be used in ELISA,IP. |
| Synonyms | ubiquitin-like modifier activating enzyme 1, UBE1X, UBE1, UBA1A, SMAX2, POC20, GXP1, CTD-2522E6.1, CFAP124, AMCX1, A1ST, A1S9T, A1S9 |
| Ig Type | Mouse IgG1 |
| Clone | 1K888 |
| Reactivity | Human |
| Specificity | Human Ubiquitin Activating Enzyme E1/UBA1 No cross-reactivity in ELISA with Insect cell lysate |
| Verified Activity | UBA1 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg K562 Whole Cell Lysate -0.5 µL anti-UBA1 mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G. -Primary antibody: -Anti-UBA1 mouse monoclonal antibody, at 1:500 dilution. -Secondary antibody: -Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 111 kDa. -Observed band size: 111 kDa |
| Application | |
| Recommended Dose | ELISA: 1:5000-1:10000; IP: 0.2-1 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human UBE1 / UBA1 (rh UBE1 / UBA1; TMPY-02840; NP_003325.2; Ser2-Arg1058). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Research Background | UBE1, also known as UBA1, belongs to the ubiquitin-activating E1 family. UBE1 gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It also catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Defects in UBA1 can cause spinal muscular atrophy X-linked type 2 (SMAX2), also known as X-linked lethal infantile spinal muscular atrophy, distal X-linked arthrogryposis multiplex congenita or X-linked arthrogryposis type 1 (AMCX1). Spinal muscular atrophy refers to a group of neuromuscular disorders characterized by degeneration of the anterior horn cells of the spinal cord, leading to symmetrical muscle weakness and atrophy. SMAX2 is a lethal infantile form presenting with hypotonia, areflexia, and multiple congenital contractures. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human UBE1 / UBA1 protein (TMPY-02840) |
| Antigen Species | Human |
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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