CD4 Protein, Human, Recombinant (His & Avi)

Name: CD4 Protein, Human, Recombinant (His & Avi)
Brand: TargetMol
SKU: TMPK-00281
Rating: 4.5 (1 reviews)

(Synonyms: EU3, CD4mut, CD4 receptor, CD4 molecule, CD4 antigen (p55), CD4) Copy Product Info

Synonyms: EU3, CD4mut, CD4 receptor, CD4 molecule, CD4 antigen (p55), CD4

Catalog No. TMPK-00281 Copy Product Info

CD4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 43.6 kDa and the accession number is P01730.

Pack Size	Price	USA Stock	Global Stock
5 µg	$41	7-10 days	7-10 days
10 µg	$62	7-10 days	7-10 days
20 µg	$98	7-10 days	7-10 days
50 µg	$188	7-10 days	7-10 days
100 µg	$313	7-10 days	7-10 days
200 µg	$568	7-10 days	7-10 days
500 µg	$1,260	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Immobilized Human CD4 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 10.6ng/ml determined by ELISA. 2. Immobilized Human CD4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 10.2ng/ml / 10.6ng/ml / 10.6ng/ml determined by ELISA.
Description	CD4 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 43.6 kDa and the accession number is P01730.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P01730
Construction	Lys26-Trp390
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	EU3, CD4mut, CD4 receptor, CD4 molecule, CD4 antigen (p55), CD4
Research Background	CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations.Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex.

Chemical Properties

Molecular Weight

43.6 kDa (predicted). Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.