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Synonyms: αVβ1, ITGAV&ITGB1, Integrin alpha V β 1, Integrin alpha V beta 1


| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $123 | 7-10 days | 7-10 days | |
| 10 µg | $189 | 7-10 days | 7-10 days | |
| 20 µg | $293 | 7-10 days | 7-10 days | |
| 50 µg | $548 | 7-10 days | 7-10 days | |
| 100 µg | $873 | 7-10 days | 7-10 days | |
| 200 µg | $1,390 | 7-10 days | 7-10 days | |
| 500 µg | $2,580 | 7-10 days | 7-10 days |
| Bioactivity | Immobilized Biotinylated Human ITGAV&ITGB1, His Avi Tag at 0.5 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-ITGAV Antibody, hFc Tag with the EC50 of 11.2 ng/ml determined by ELISA (QC Test). |
| Description | Integrin alpha V beta 1 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P06756-1(ITGAV)&P05556-1(ITGB1). |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P06756-1(ITGAV)&P05556-1(ITGB1) |
| Construction | Phe31-Val992 (ITGAV) acidic tail and Gln21-Asp728 (ITGB1) basic tail |
| Protein Purity | > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC |
| Endotoxin | < 1 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, 8% trehalose (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | αVβ1, ITGAV&ITGB1, Integrin alpha V β 1, Integrin alpha V beta 1 |
| Research Background | Integrins are transmembrane heterodimeric receptors that contribute to diverse biological functions and play a conserved role in mediating cell adhesion to fixed extracellular ligands and in the maintenance of tissue integrity. αVβ1 is the major integrin on several different primary fibroblasts responsible for binding to TGFb1 LAP and for mediating activation of latent TGFb by these cells. |
| Molecular Weight | 114 kDa (ITGAV) and 83.2 kDa (ITGB1) (Predicted); 140-170 kDa (ITGAV) and 105-135 kDa (ITGB1) (Reducing condition, due to glycosylation) |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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