Ephrin A5/EFNA5 Protein, Rhesus, Recombinant (hFc)

Measured by its binding ability in a functional ELISA. Immobilized Rhesus EFNA5-Fc at 10 μg/mL (100 μl/well) can bind biotinylated Rhesus EPHA4 . The EC50 of biotinylated Rhesus EPHA4 is 9.3-21.6 ng/mL.

Ephrin A5/EFNA5 Protein, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 48.2 kDa and the accession number is A0A8J8Y8X9.

Rhesus

HEK293 Cells

> 92 % as determined by SDS-PAGE

Ephrin-A5 also known as EFNA5, is a member of the Ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: ephrin-A ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A5/EFNA5 may function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Ephrin-A5/EFNA5 also serves as a cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion.