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Anti-2B4/CD244 Antibody (1B831)

😃Good
Catalog No. TMAY-00155
Alias SLAMF4, Nmrk, NKR2B4, NAIL, CD244 molecule, natural killer cell receptor 2B4, 2B4

Anti-2B4/CD244 Antibody (1B831) is a Rabbit antibody targeting 2B4/CD244. Anti-2B4/CD244 Antibody (1B831) can be used in WB,ELISA(Cap),IP.

Anti-2B4/CD244 Antibody (1B831)

Anti-2B4/CD244 Antibody (1B831)

😃Good
Catalog No. TMAY-00155Alias SLAMF4, Nmrk, NKR2B4, NAIL, CD244 molecule, natural killer cell receptor 2B4, 2B4
Anti-2B4/CD244 Antibody (1B831) is a Rabbit antibody targeting 2B4/CD244. Anti-2B4/CD244 Antibody (1B831) can be used in WB,ELISA(Cap),IP.
Pack SizePriceAvailabilityQuantity
20 μL$93 7-10 days
100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Anti-2B4/CD244 Antibody (1B831) is a Rabbit antibody targeting 2B4/CD244. Anti-2B4/CD244 Antibody (1B831) can be used in WB,ELISA(Cap),IP.
AliasSLAMF4, Nmrk, NKR2B4, NAIL, CD244 molecule, natural killer cell receptor 2B4, 2B4
Ig Type
Monoclonal Rabbit IgG
Clone
1B831
Reactivity
Human
Verified Activity
1. Anti-2B4 rabbit monoclonal antibody at 1:500 dilution.

-Lane A: Daudi Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:42 kDa.

-Observed band size:28 kDa(We are unsure as to the identity of these extra bands.)

2. 2B4 was immunoprecipitated using:

-Lane A:0.5 mg Daudi Whole Cell Lysate.

-2 µL anti-2B4 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

-Primary antibody:

-Anti-2B4 rabbit monoclonal antibody, at 1:200 dilution.

-Secondary antibody:

-Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution.

-Developed using the odyssey technique.

-Performed under reducing conditions.

-Predicted band size: 42 kDa.

-Observed band size: 28 kDa
Application
WB,ELISA(Cap),IP
Recommended Dose
WB: 1:500-1:2000; IP: 1-4 μL/mg of lysate; ELISA(Cap): 1:250-1:2000
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human 2B4 / CD244 (rh 2B4 / CD244; TMPY-01568; NP_057466.1; Met1-Arg221).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundThe CD244 antigen, also known as 2B4, is a cell surface glycoprotein implicated in the regulation of natural killer and T lymphocyte function. 2B4 is a member of the signaling lymphocyte activation molecule (SLAM)-related receptor family and is important for stimulating NK cell cytotoxicity and cytokine production, which is expressed on all NK cells, a subpopulation of T cells, monocytes and basophils. The 2B4 antigen identified on NK cells and T cells is capable of transmitting stimulatory signals and non-MHC-restricted killing. Reported as an activating receptor, human 2B4 can effectively activate and enhance NK cell–mediated cytotoxicity, induce secretion of IFN-γ and matrix metalloproteinases (MMPs), as well as NK cell invasiveness. As a cell surface glycoprotein of the Ig-superfamily structurally related to CD2-like molecules such as CD2, CD48, CD58, CD84, Ly-9, and SLAM, 2B4 (CD244) is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. 2B4 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Human 2B4 / CD244 protein (TMPY-01568)
Antigen Species
Human
Biology Area
Cancer Drug Targets, ITIM/ITAM Immunoreceptors and Related Molecules
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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