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Anti-BCL2 Antibody (9E869)
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Catalog No. TMAH-00107
Alias PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (9E869) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (9E869) can be used in ELISA, WB, FCM.
Anti-BCL2 Antibody (9E869)
Anti-BCL2 Antibody (9E869)
😃Good
Catalog No. TMAH-00107Alias PPP1R50, Bcl-2, B-cell CLL/lymphoma 2
Anti-BCL2 Antibody (9E869) is a Mouse antibody targeting BCL2. Anti-BCL2 Antibody (9E869) can be used in ELISA, WB, FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, FCM Reactivity: Human, Mouse |
| Alias | PPP1R50, Bcl-2, B-cell CLL/lymphoma 2 |
| Ig Type | IgG1 |
| Clone | 9E869 |
| Reactivity | Human, Mouse |
| Verified Activity | 1. Western Blot -Positive WB detected in: BCL2 antibody at 1:1000 -Lane 1: Hela whole cell lysate -Lane 2: THP-1 whole cell lysate -Lane 3: NIH/3T3 whole cell lysate -Lane 4: MCF-7 whole cell lysate -Secondary: Goat polyclonal to Mouse IgG at 1/20000 dilution -Predicted band size: 26KDa -Observed band size: 26 KDa -Exposure time: 5min 2. Overlay Peak curve showing Jurkat cells stained with TMAH-00107 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, FCM |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Research Background | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human poptosis regulator Bcl-2 Protein (2-211AA) |
| Antigen Species | Human |
| Gene ID | 596 |
| Uniprot ID | |
| Biology Area | Cell Biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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