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Anti-4EBP1 Antibody (9E210)
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Catalog No. TMAY-00090
Alias PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1
Anti-4EBP1 Antibody (9E210) is a Mouse antibody targeting 4EBP1. Anti-4EBP1 Antibody (9E210) can be used in WB,ELISA,ICC/IF.
Anti-4EBP1 Antibody (9E210)
Anti-4EBP1 Antibody (9E210)
😃Good
Catalog No. TMAY-00090Alias PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1
Anti-4EBP1 Antibody (9E210) is a Mouse antibody targeting 4EBP1. Anti-4EBP1 Antibody (9E210) can be used in WB,ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $198 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA, ICC/IF Reactivity: Human |
| Alias | PHAS-I, eukaryotic translation initiation factor 4E binding protein 1, BP-1, 4E-BP1, 4EBP1 |
| Ig Type | Mouse IgG1 |
| Clone | 9E210 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human 4EBP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human 4EBP1 monoclonal antibody (1:300) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus and cytoplasm. 2. Anti-4EBP1 mouse monoclonal antibody at 1:500 dilution. -Lane A: A431 Whole Cell Lysate. -Lane B: K562 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:13 kDa. -Observed band size:13 kDa |
| Application | WB,ELISA,ICC/IF |
| Recommended Dose | WB: 1:500-1:1000; ELISA: 1:5000-1:10000; ICC-IF: 1:100-1:500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 4E-BP1 / EIF4EBP1 (rh 4E-BP1 / EIF4EBP1; TMPY-01769; Ser 2-Ile 118; NP_004086.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography . |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The translational suppressor eIF4E binding protein-1, 4E-BP1 functions as a key regulator in cellular growth, differentiation, apoptosis and survival. The Eif4ebp1 gene, encoding 4E-BP1, is a direct target of a transcription factor activating transcription factor-4 (ATF4), a master regulator of gene expression in stress responses. 4E-BP1 is characterized by its capacity to bind specifically to eIF4E and inhibit its interaction with eIF4G. Phosphorylation of 4E-BP1 regulates eIF4E availability, and therefore, cap-dependent translation, in cell stress. Binding of eIF4E to eIF4G is inhibited in a competitive manner by 4E-BP1. Phosphorylation of 4E-BP1 decreases the affinity of this protein for eIF4E, thus favouring the binding of eIF4G and enhancing translation. 4E-BP1 is important for beta-cell survival under endoplasmic reticulum (ER) stress. 4E-BP1 mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recently, 4E-BP1 was found to be a key factor, which converges several oncogenic signals, phosphorylates the molecules, and drives the downstream proliferative signals. Recent studies showed that high expression of phosphorylated 4E-BP-1 (p-4E-BP1) is associated with poor prognosis, tumor progression, or nodal metastasis in different human cancers. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human 4E-BP1 / EIF4EBP1 Protein (TMPY-01769) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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