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TNF alpha Protein, Cynomolgus, Recombinant, Biotinylated

(Synonyms: tumor necrosis factor, TNF-α) Copy Product Info

Synonyms: tumor necrosis factor, TNF-α

Catalog No. TMPY-05516 Copy Product Info
TNF alpha Protein, Cynomolgus, Recombinant, Biotinylated is expressed in E. coli expression system. The predicted molecular weight is 17.4 kDa and the accession number is P79337.

Product Introduction

Bioactivity
Bioactivity
1.Immobilized Anti-TNFa (Adalimumab Biosimilar) at 5 μg/mL (100 μL/well) can bind Recombinant Cynomolgus TNF-alpha/TNFA Protein, Biotinylated (Cat#TMPY-05516), the EC50 is 2.1-6.2 ng/mL (Routinely tested).
2.Immobilized Recombinant Cynomolgus/Rhesus TNFR2/CD120b/TNFRSF1B Protein (Fc Tag) (Cat#TMPY-03588) at 5 μg/mL (100 μL/well) can bind Recombinant Cynomolgus TNF-alpha/TNFA Protein, Biotinylated (Cat#TMPY-05516), the EC50 is 3.7-11.2 ng/mL (QC tested).
Description
TNF alpha Protein, Cynomolgus, Recombinant, Biotinylated is expressed in E. coli expression system. The predicted molecular weight is 17.4 kDa and the accession number is P79337.
Species
Cynomolgus
Expression System
E. coli
TagTag Free
Accession NumberP79337
ConstructionA DNA sequence encoding the cynomolgus TNF (NP_001272206.1) (Val77-Leu233) was expressed and purified with an initial Met. The purified protein was biotinylated in vitro. Predicted N terminal: Met
Protein Purity
> 95 % as determined by SDS-PAGE.
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Synonymstumor necrosis factor, TNF-α
Research Background
Chemical Properties
Molecular Weight17.4 kDa (predicted)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: TNF alpha Protein, Cynomolgus, Recombinant, Biotinylated chemical structure | TNF alpha Protein, Cynomolgus, Recombinant, Biotinylated molecular weight