5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated

Name: 5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated
Brand: TargetMol
SKU: TMPJ-00268
Rating: 4.5 (1 reviews)

(Synonyms: WAIF1, TPBG, M6P1, 5T4AG, 5T4) Copy Product Info

Synonyms: WAIF1, TPBG, M6P1, 5T4AG, 5T4

Catalog No. TMPJ-00268 Copy Product Info

5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 60-90 KDa and the accession number is Q13641.

Pack Size	Price	USA Stock	Global Stock
5 µg	$129	7-10 days	7-10 days
10 µg	$213	7-10 days	7-10 days
20 µg	$355	7-10 days	7-10 days
50 µg	$625	7-10 days	7-10 days
100 µg	$963	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Loaded Human TPBG Antibody (5G9) (Human IgG1) on Protein-A Biosensor, can bind Biotinylated Human 5T4 (C-Avi-6His) with an affinity constant of 9.29 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human TPBG Antibody (5G9) (Human IgG1) at 2μg/ml (100 μl/well) can bind Biotinylated Human TPBG-Avi-His. The ED50 of Human Biotinylated TPBG-Avi-His is 0.13 ng/ml. (Regularly tested)
Description	5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 60-90 KDa and the accession number is Q13641.
Species	Human
Expression System	HEK293 Cells
Tag	C-Avi-6xHis
Accession Number	Q13641
Amino Acid	Ser32-Ser355
Construction	Ser32-Ser355
Protein Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Synonyms	WAIF1, TPBG, M6P1, 5T4AG, 5T4
Research Background	Trophoblast glycoprotein, also known as TPBG and 5T4, it belongs to the LRR family of proteins. The C-terminus of LRR N-terminal cap (LRRNT) and LRR 1 are essential for the inhibition of the Wnt signaling pathway. TPBG may function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization. It expressed by all types of trophoblasts as early as 9 weeks of development. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Chemical Properties

Molecular Weight

60-90 KDa (reducing condition)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Dose Conversion

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Keywords

WAIF-1WAIF 1

Related Tags: 5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated chemical structure | 5T4/TPBG Protein, Human, Recombinant (Avi & His), Biotinylated molecular weight