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Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600)
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Catalog No. TMAY-01562
Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600) is a Mouse antibody targeting Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA. Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600) can be used in WB,ELISA.
Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600)
Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600)
😃Good
Catalog No. TMAY-01562
Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600) is a Mouse antibody targeting Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA. Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600) can be used in WB,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $293 | 7-10 days |
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Bioactivity
Related Conjugates and Formulations
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Chemical Properties
| Description | Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600) is a Mouse antibody targeting Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA. Anti-Influenza A H1N1 (Swine Flu 2009) Hemagglutinin/HA Antibody (9S600) can be used in WB,ELISA. |
| Ig Type | Monoclonal Mouse IgG2b |
| Clone | 9S600 |
| Verified Activity | High Five cells were infected by Bac-HA, cells were collected at 48 hours postinfection, and the cell surface expression of HA were measured by flow cytometry. 106Cells were stained with 1 μg Purified Mouse Anti-H1N1-HA antibody for 20 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry. |
| Application | WB,ELISA |
| Recommended Dose | WB: his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect H1N1 HA in WB.; ELISA: 1:1000-1:2000; FCM: 1:25-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H1N1 hemagglutinin (HA) extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant H1N1 HA protein |
| Antigen Species | H1N1 |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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