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IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi)
(Synonyms: IL-10RA, IL10RA, IL-10R1, IL-10R subunit 1, IL10R, IL-10 R α, IL-10 R alpha, IBD28, HIL-10R, CDw210a) Copy Product InfoSynonyms: IL-10RA, IL10RA, IL-10R1, IL-10R subunit 1, IL10R, IL-10 R α, IL-10 R alpha, IBD28, HIL-10R, CDw210a
Catalog No. TMPK-00334 Copy Product Info
IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 27 kDa and the accession number is Q13651.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $58 | 7-10 days | 7-10 days | |
| 10 µg | $92 | 7-10 days | 7-10 days | |
| 20 µg | $148 | 7-10 days | 7-10 days | |
| 50 µg | $289 | 7-10 days | 7-10 days | |
| 100 µg | $487 | 7-10 days | 7-10 days | |
| 200 µg | $883 | 7-10 days | 7-10 days | |
| 500 µg | $1,950 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Immobilized Human IL-10, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human IL-10 R alpha, His Tag with the EC50 of 21.8ng/ml determined by ELISA (QC Test).
2. Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Human IL-10, His Tag with an affinity constant of 0.13 nM as determined in SPR assay. |
| Description | IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 27 kDa and the accession number is Q13651. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | Q13651 |
| Construction | His22-Asn235 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | IL-10RA, IL10RA, IL-10R1, IL-10R subunit 1, IL10R, IL-10 R α, IL-10 R alpha, IBD28, HIL-10R, CDw210a |
| Research Background | The interleukin-10 receptor alpha (IL10RA) gene codes for the alpha chain of the IL-10 receptor which binds the cytokine IL-10. IL-10 is an anti-inflammatory cytokine with immunoregulatory function during the pathogenesis of many inflammatory disorders in livestock, including Johne's disease (JD). JD is a chronic enteritis in cattle caused by Mycobacterium avium subsp. paratuberculosis (MAP) and is responsible for significant economic losses to the dairy industry. Several candidate genes including IL10RA have been found to be associated with JD. |
| Molecular Weight | 27 kDa (predicted). Due to glycosylation, the protein migrates to 40-48 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi) chemical structure | IL-10R alpha/IL-10RA Protein, Human, Recombinant (His & Avi) molecular weight
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