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Anti-PTP1B Antibody (5M68)
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Catalog No. TMAY-00587
Alias PTPN1, PTP1B, protein tyrosine phosphatase, non-receptor type 1
Anti-PTP1B Antibody (5M68) is a Rabbit antibody targeting PTP1B. Anti-PTP1B Antibody (5M68) can be used in WB,ELISA,IP.
Anti-PTP1B Antibody (5M68)
Anti-PTP1B Antibody (5M68)
😃Good
Catalog No. TMAY-00587Alias PTPN1, PTP1B, protein tyrosine phosphatase, non-receptor type 1
Anti-PTP1B Antibody (5M68) is a Rabbit antibody targeting PTP1B. Anti-PTP1B Antibody (5M68) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $270 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: WB, ELISA, IP Reactivity: Human |
| Alias | PTPN1, PTP1B, protein tyrosine phosphatase, non-receptor type 1 |
| Ig Type | Rabbit IgG |
| Clone | 5M68 |
| Reactivity | Human |
| Verified Activity | 1. Anti-PTPN1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: Hela whole cell Lysate. -Lane B: MCF7 whole cell Lysate. -Lane C: A549 whole cell Lysate. -Lane D: Jurkat whole cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:50 kDa. -Observed band size:53 kDa. 2. PTPN1 was immunoprecipitated using: -Lane A:0.5 mg A549 Whole Cell Lysate. -2 µL anti-PTPN1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-PTPN1 rabbit monoclonal antibody, at 1:330 dilution. -Secondary antibody: -Clean-Blotô IP Detection Reagent (HRP) at 1:1000 dilution. -Developed using the DAB staining technique. -Performed under reducing conditions. -Predicted band size: 50 kDa. -Observed band size: 50 kDa. 3. Anti-PTPN1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: PTPN1 konckout Hela Cell Lysate. -Lane B: Hela Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L /HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:50 kDa. -Observed band size:53 kDa(Validation Experiment) |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human PTP1B / PTPN1 (rh PTP1B / PTPN1; TMPY-04125; NP_002818.1; Glu 2-Asn 321). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | PTP1B, also known as PTPN1, belongs to the protein-tyrosine phosphatase (PTP) family. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTP1B contains 1 tyrosine-protein phosphatase domain and is expressed in many tissues. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. PTP1B was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of PTP1B in cell growth control, and cell response to IFN stimulation. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human PTP1B / PTPN1 protein (TMPY-04125) |
| Antigen Species | Human |
| Biology Area | Phosphatases and Regulators |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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