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Anti-CALML5 Antibody (2A846)

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Catalog No. TMAY-02056
Alias CLSP, calmodulin like 5

Anti-CALML5 Antibody (2A846) is a Rabbit antibody targeting CALML5. Anti-CALML5 Antibody (2A846) can be used in ELISA,FCM,ICC/IF.

Anti-CALML5 Antibody (2A846)

Anti-CALML5 Antibody (2A846)

😃Good
Catalog No. TMAY-02056Alias CLSP, calmodulin like 5
Anti-CALML5 Antibody (2A846) is a Rabbit antibody targeting CALML5. Anti-CALML5 Antibody (2A846) can be used in ELISA,FCM,ICC/IF.
Pack SizePriceAvailabilityQuantity
20 μL$92 7-10 days
100 μL$269 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Monoclonal

Application: ELISA, FCM, ICC/IF

Reactivity: Human
AliasCLSP, calmodulin like 5
Ig Type
Rabbit IgG
Clone
2A846
Reactivity
Human
Verified Activity
1. Immunofluorescence staining of Human CALML5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CALML5 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
2. Flow cytometric analysis of Human CALML5 expression on A431 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CALML5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Application
ELISA,FCM,ICC/IF
Recommended Dose
ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human CALML5 (rh CALML5; TMPY-01953; AAH39172.1; Met 1-Glu 146).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundCalmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5 / CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5 / CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5 / CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5 / CLSP have been characterized by NMR spectroscopy. The mobility of CALML5 / CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human CALML5 protein (TMPY-01953)
Antigen Species
Human
Chemical Properties
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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