Anti-ACAA2 Antibody (7X361) is an antibody targeting ACAA2. Anti-ACAA2 Antibody (7X361) can be used in ELISA, WB, IHC, IF.

Rabbit IgG

7X361

Human, Mouse

1. Western Blot
-Positive WB detected in: Hela whole cell lysate(20µg), A549 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), 293T whole cell lysate(20µg), MCF7 whole cell lysate(20µg), JK whole cell lysate(20µg), Mouse Liver tissue lysate(20µg), Mouse Kidney tissue lysate(20µg)
-All lanes: ACAA2 antibody at 1:1000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 42 kDa
-Observed band size: 42 kDa
-Exposure time: 10s
2. IHC image of TMAH-00004 diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
3. IHC image of TMAH-00004 diluted at 1:150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
4. IHC image of TMAH-00004 diluted at 1:150 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
5. Immunofluorescence staining of Hela cell with TMAH-00004 at 1:12, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

ELISA, WB, IHC, IF

WB:1:500-1:2000; IHC:1:50-1:200; IF:1:50-1:200.

Monoclonal

Unconjugated

A synthetic peptide: Human ACAA2

Human

10449

Cardiovascular, Tags & Cell Markers, Metabolism, Signal transduction