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Anti-FASN Polyclonal Antibody is a Rabbit antibody targeting FASN. Anti-FASN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $222 | 7-10 days | |
100 μL | $373 | 7-10 days |
Description | Anti-FASN Polyclonal Antibody is a Rabbit antibody targeting FASN. Anti-FASN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
Alias | SDR27X1, OA-519, fatty acid synthase, FAS |
Ig Type | IgG |
Reactivity | Human,Rat (predicted:Mouse,Sheep) |
Verified Activity | 1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-FASN Polyclonal Antibody, Unconjugated (TMAB-00659) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. 2. Sample: Lane 1: Human HeLa cell lysates Lane 2: Human HepG2 cell lysates Primary: Anti-FASN (TMAB-00659) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 272 kDa Observed band size: 250 kDa |
Application | WB,IHC-P,IHC-Fr,IF,ELISA |
Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
Antibody Type | Polyclonal |
Host Species | Rabbit |
Subcellular Localization | Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Construction | Hybridoma Polyclonal Antibody |
Purification | Protein A purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | FASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation. |
Immunogen | KLH conjugated synthetic peptide: humanFASN |
Antigen Species | Human |
Gene ID | |
Uniprot ID | |
Biology Area | Integration of energy metabolism,Metabolism of lipids and lipoproteins,Enzymes,Synthesis,Energy Metabolism,Integration of energy,Lipid metabolism,Fatty acid oxidation,Cancer,Obesity,Metabolism,Energy Metabolism,Lipid metabolism |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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