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Anti-FASN Polyclonal Antibody

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Catalog No. TMAB-00659
Alias SDR27X1, OA-519, fatty acid synthase, FAS

Anti-FASN Polyclonal Antibody is a Rabbit antibody targeting FASN. Anti-FASN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-FASN Polyclonal Antibody

Anti-FASN Polyclonal Antibody

😃Good
Catalog No. TMAB-00659Alias SDR27X1, OA-519, fatty acid synthase, FAS
Anti-FASN Polyclonal Antibody is a Rabbit antibody targeting FASN. Anti-FASN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$2227-10 days
100 μL$3737-10 days
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Product Introduction

Bioactivity
Description
Anti-FASN Polyclonal Antibody is a Rabbit antibody targeting FASN. Anti-FASN Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
AliasSDR27X1, OA-519, fatty acid synthase, FAS
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Sheep)
Verified Activity
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-FASN Polyclonal Antibody, Unconjugated (TMAB-00659) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: Human HeLa cell lysates
Lane 2: Human HepG2 cell lysates
Primary: Anti-FASN (TMAB-00659) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 272 kDa
Observed band size: 250 kDa
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundFASN (Fatty Acid Synthase) is a central enzyme in de novo lipogenesis. FAS is a target for SREBP and is upregulated by LXR activation; it is also one of the accepted markers for insulin resistance, SREBP and LXR activation.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: humanFASN
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
Integration of energy metabolism,Metabolism of lipids and lipoproteins,Enzymes,Synthesis,Energy Metabolism,Integration of energy,Lipid metabolism,Fatty acid oxidation,Cancer,Obesity,Metabolism,Energy Metabolism,Lipid metabolism
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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