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Anti-CDC5L Antibody (9X496)
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Catalog No. TMAH-00242
Alias Pombe Cdc5 related protein, PCDC5RP, KIAA0432, dJ319D22.1, Cell division cycle 5-like protein, Cell division cycle 5 S. pombe homolog, CEF1, CDC5-LIKE, Cdc5 related protein, CDC5 cell division cycle 5-like (S. pombe), CDC5
Anti-CDC5L Antibody (9X496) is an antibody targeting CDC5L. Anti-CDC5L Antibody (9X496) can be used in ELISA, WB, IHC, IF, IP.
Anti-CDC5L Antibody (9X496)
Anti-CDC5L Antibody (9X496)
😃Good
Catalog No. TMAH-00242Alias Pombe Cdc5 related protein, PCDC5RP, KIAA0432, dJ319D22.1, Cell division cycle 5-like protein, Cell division cycle 5 S. pombe homolog, CEF1, CDC5-LIKE, Cdc5 related protein, CDC5 cell division cycle 5-like (S. pombe), CDC5
Anti-CDC5L Antibody (9X496) is an antibody targeting CDC5L. Anti-CDC5L Antibody (9X496) can be used in ELISA, WB, IHC, IF, IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $347 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CDC5L Antibody (9X496) is an antibody targeting CDC5L. Anti-CDC5L Antibody (9X496) can be used in ELISA, WB, IHC, IF, IP. |
| Alias | Pombe Cdc5 related protein, PCDC5RP, KIAA0432, dJ319D22.1, Cell division cycle 5-like protein, Cell division cycle 5 S. pombe homolog, CEF1, CDC5-LIKE, Cdc5 related protein, CDC5 cell division cycle 5-like (S. pombe), CDC5 |
| Ig Type | Rabbit IgG |
| Clone | 9X496 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate -All lanes: CDC5L antibody at 1.3μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 93 KDa -Observed band size: 100 KDa 2. IHC image of TMAH-00242 diluted at 1:126.5 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 3. IHC image of TMAH-00242 diluted at 1:126.5 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. 4. Immunofluorescence staining of A549 cells with TMAH-00242 at 1:42, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). 5. Immunoprecipitating CDC5L in Hela whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00242 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00242 (3μg) + Hela whole cell lysate (500μg) -Lane 3: Hela whole cell lysate (20μg) |
| Application | ELISA, WB, IHC, IF, IP |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200; IP:1:200-1:1000. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Nucleus speckle. Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | DNA-binding protein involved in cell cycle control. May act as a transcription activator. Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR). |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human CDC5L |
| Antigen Species | Human |
| Gene ID | 988 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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