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FAP Protein, Human, Recombinant (His)
(Synonyms: SIMP, fibroblast activation protein, α, fibroblast activation protein, alpha, Fibroblast Activation Protein α, Fibroblast Activation Protein alpha, Fapα, Fapalpha, FAPA, DPPIVA, DPPIV) Copy Product InfoSynonyms: SIMP, fibroblast activation protein, α, fibroblast activation protein, alpha, Fibroblast Activation Protein α, Fibroblast Activation Protein alpha, Fapα, Fapalpha, FAPA, DPPIVA, DPPIV
Catalog No. TMPY-02154 Copy Product Info
FAP Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 87.2 kDa and the accession number is Q12884-1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $97 | - | In Stock | |
| 10 μg | $158 | - | In Stock | |
| 20 μg | $259 | - | In Stock | |
| 50 μg | $517 | 7-10 days | 7-10 days | |
| 100 μg | $800 | 7-10 days | 7-10 days | |
| 200 μg | $1,230 | 7-10 days | 7-10 days | |
| 500 μg | $2,200 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
Batch Information
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Purity:SDS-PAGE: 96.20%; SEC-HPLC: 94%
Appearance:Solution
Color:Transparent
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | 1. Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >1200 pmol/min/μg. 2. Loaded anti-FAP antibody, human IgG1 on proA Biosensor, can bind FAP Protein, Human, Recombinant (His) with an affinity constant of 0.396 nM as determined in BLI assay (Sartorius Octet RED384). 3. Immobilized FAP Protein, Human, Recombinant (His) at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, human IgG1, the EC50 is 1.0-4.0 ng/mL. |
| Description | FAP Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 87.2 kDa and the accession number is Q12884-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | N-His |
| Accession Number | Q12884-1 |
| Construction | A DNA sequence encoding the human FAP (Q12884-1) extracellular domain (Leu26-Asp760) was fused with the polyhistidie tag at the N-terminus. Predicted N terminal: His |
| Protein Purity | > 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.   |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Supplied as sterile 25 mM Tris, 250 mM NaCl, pH 8.2. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | Proteins are shipped with blue ice. |
| Synonyms | SIMP, fibroblast activation protein, α, fibroblast activation protein, alpha, Fibroblast Activation Protein α, Fibroblast Activation Protein alpha, Fapα, Fapalpha, FAPA, DPPIVA, DPPIV |
| Research Background |
| Molecular Weight | 87.2 kDa (predicted); 90 kDa (reducing condition, due to glycosylation) |
| Storage | Store at low temperature Store at -80°C |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: FAP Protein, Human, Recombinant (His) chemical structure | FAP Protein, Human, Recombinant (His) molecular weight
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