G-CSF Protein, Mouse, Recombinant

Name: G-CSF Protein, Mouse, Recombinant
Brand: TargetMol
SKU: TMPY-05636
Price: 129 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: MGI-IG, G-CSF, Csfg, colony stimulating factor 3 (granulocyte)) Copy Product Info

Synonyms: MGI-IG, G-CSF, Csfg, colony stimulating factor 3 (granulocyte)

Catalog No. TMPY-05636 Copy Product Info

G-CSF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19 kDa and the accession number is P09920.

Pack Size	Price	USA Stock	Global Stock
5 µg	$129	-	In Stock
10 µg	$212	-	In Stock
20 µg	$353	In Stock	In Stock
50 µg	$490	-	In Stock
100 µg	$653	-	In Stock
200 µg	$852	-	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Batch Information

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Purity:> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.

Resource Download

COA User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	1. Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 40-160 pg/mL. 2. Immobilized G-CSF Protein, Mouse, Recombinant, HPLC-verified at 2 μg/ml (100 μl/well) can bind CSF3R/G-CSFR Protein, Human, Recombinant (hFc Tag), The EC50 is 7-38 ng/mL.
Description	G-CSF Protein, Mouse, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 19 kDa and the accession number is P09920.
Species	Mouse
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	P09920
Construction	A DNA sequence encoding the mouse CSF3 (NP_034101.1) (Met1-Ala208) was expressed and purified. Predicted N terminal: Val 31
Protein Purity	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. ,
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	MGI-IG, G-CSF, Csfg, colony stimulating factor 3 (granulocyte)
Research Background	Granulocyte-colony stimulating factor (G-CSF) is a growth factor and an essential cytokine belonging to the CSF family of hormone-like glycoproteins. It is produced by numerous cell types including immune and endothelial cells. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. Recent animal studies have also revealed that G-CSF activates multiple signaling pathways, such as Akt and also the Janus family kinase-2 and signal transducer and activation of transcription-3 (Jak2-STAT3) pathway, thereby promoting survival, proliferation, differentiation and mobilisation of haematopoietic stem and progenitor cells. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent. G-CSF prevents left ventricular remodeling after myocardial infarction by decreasing cardiomyocyte death and by increasing the number of blood vessels, suggesting the importance of direct actions of G-CSF on the myocardium rather than through mobilization and differentiation of stem cells. Accordingly, recombinant human (rh)G-CSF has been extensively used in clinical haematology and oncology to enable bone marrow transplantation or to treat chemotherapy-associated neutropenia. In preclinical study, G-CSF improved cardiac function and perfusion by angiomyogenesis and protection of cardiomyocytes in myocardial infarction.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

19 kDa (predicted)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.