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Anti-Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein Antibody (6O671) is a Mouse antibody targeting Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein. Anti-Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein Antibody (6O671) can be used in WB,ELISA.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μL | $93 | 7-10 days | |
100 μL | $294 | 7-10 days |
Description | Anti-Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein Antibody (6O671) is a Mouse antibody targeting Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein. Anti-Zika virus (ZIKV) (strain Zika SPH2015) E/Envelope protein Antibody (6O671) can be used in WB,ELISA. |
Ig Type | Monoclonal Mouse IgG1 |
Clone | 6O671 |
Verified Activity | Anti-Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E / Envelope protein (Domain III) mouse monoclonal antibody at 1:1000 dilution -Sample:Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E / Envelope protein (Domain III,His Tag ) -Lane A: 30ng -Lane B: 10ng -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. |
Application | WB,ELISA |
Recommended Dose | WB: 1:1000-1:5000; ELISA: 1:1000-1:2000 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E / Envelope Protein ( TMPY-04868; ALU33341.1; Val593-Lys699). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Purification | Protein A |
Appearance | Liquid |
Formulation | 0.2 μm filtered solution in PBS |
Research Background | Envelope of Zika virus is responsible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent with those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross-reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross-reactivity with dengue and YF.Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus. |
Conjucates | Unconjugated |
Immunogen | Recombinant Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-E / Envelope Protein Protein (TMPY-04868) |
Antigen Species | ZIKV |
Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
Transport | Shipping with blue ice. |
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