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Anti-Phospho-NFKBIA (Ser32) Antibody (5H611)
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Catalog No. TMAH-00936
Alias Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) is an antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) can be used in ELISA, WB, IF.
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611)
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611)
😃Good
Catalog No. TMAH-00936Alias Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) is an antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | 197 € | 7-10 days | |
| 100 μL | 331 € | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) is an antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) can be used in ELISA, WB, IF. |
| Alias | Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBI, MAD-3, IKBA, IkB α |
| Ig Type | Rabbit IgG |
| Clone | 5H611 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in A549 whole cell lysate(treated with Calyculin A or not) -All lanes Phospho-NFKBIA antibody at 1.07μg/ml -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 39 KDa -Observed band size: 39 KDa 2. Immunofluorescence staining of A549 cells with TMAH-00936 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human Phospho-NFKBIA (S32) |
| Antigen Species | Human |
| Gene ID | 4792 |
| Uniprot ID | |
| Biology Area | Signal Transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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