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Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) (Synonyms: p-NFKBIA (Ser32), p-NFKBIA (S32), Phospho-NFKBIA (S32)...)

Catalog No. TMAH-00936 Copy Product Info
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Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) is an antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) can be used in ELISA,IF,WB.

Anti-Phospho-NFKBIA (Ser32) Antibody (5H611)

Copy Product Info
🥰Excellent
Catalog No. TMAH-00936
Synonyms p-NFKBIA (Ser32), p-NFKBIA (S32), Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBIA (p-Ser32), NFKBIA (p-S32), NFKBI, MAD-3, IKBA, IkB α

Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) is an antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) can be used in ELISA,IF,WB.

Anti-Phospho-NFKBIA
(Ser32) Antibody
(5H611)
Pack SizePriceUSA StockGlobal StockQuantity
50 μL$2087-10 days7-10 days
100 μL$3497-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Description
Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) is an antibody targeting Phospho-NFKBIA (Ser32). Anti-Phospho-NFKBIA (Ser32) Antibody (5H611) can be used in ELISA,IF,WB.
Synonymsp-NFKBIA (Ser32), p-NFKBIA (S32), Phospho-NFKBIA (S32), nuclear factor of κ light polypeptide gene enhancer in B-cells inhibitor, alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, α, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha, NFKBIA (p-Ser32), NFKBIA (p-S32), NFKBI, MAD-3, IKBA, IkB α
Ig Type
Rabbit IgG
Clone
5H611
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in A549 whole cell lysate(treated with Calyculin A or not)
-All lanes Phospho-NFKBIA antibody at 1.07μg/ml
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 39 KDa
-Observed band size: 39 KDa
2. Immunofluorescence staining of A549 cells with TMAH-00936 at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Application
Recommended Dose
WB:1:500-1:5000; IF:1:20-1:200.
Antibody Type
Monoclonal
Subcellular LocalizationCytoplasm. Nucleus.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundInhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human Phospho-NFKBIA (S32)
Antigen Species
Human
Gene Name
NFKBIA
Gene ID
Uniprot ID
Biology Area
Signal Transduction
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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