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VEGFR2/KDR Protein, Human, Recombinant (aa 20-764, His & Avi), Biotinylated

(Synonyms: VEGFR-2, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor flk-1, Kinase insert domain receptor, KDR, FLK-1, Fetal liver kinase 1) Copy Product Info

Synonyms: VEGFR-2, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor flk-1, Kinase insert domain receptor, KDR, FLK-1, Fetal liver kinase 1

Catalog No. TMPJ-00414 Copy Product Info
VEGFR2/KDR Protein, Human, Recombinant (aa 20-764, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 120-150 KDa and the accession number is P35968.

Product Introduction

Bioactivity
Bioactivity
1. Loaded Anti-Human VEGFR2 mAb-Fc on Protein A Biosensor, can bind Biotinylated Human VEGFR2-6His-Avi with an affinity constant of 1.1 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Biotinylated Human VEGFR2-His-Avi at 1μg/ml (100 μl/well)can bind Anti-Human VEGFR2 mAb. The ED50 of Anti-Human VEGFR2 mAb is 44.08 ng/ml. (Regularly tested)
Description
VEGFR2/KDR Protein, Human, Recombinant (aa 20-764, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 120-150 KDa and the accession number is P35968.
Species
Human
Expression System
HEK293 Cells
TagC-6xHis-Avi
Accession NumberP35968
Amino AcidAla20-Glu764
ConstructionAla20-Glu764
Protein Purity
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxin< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & StorageLyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsVEGFR-2, Vascular endothelial growth factor receptor 2, Protein-tyrosine kinase receptor flk-1, Kinase insert domain receptor, KDR, FLK-1, Fetal liver kinase 1
Research Background
Chemical Properties
Molecular Weight120-150 KDa (reducing condition)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

Related Tags: VEGFR2/KDR Protein, Human, Recombinant (aa 20-764, His & Avi), Biotinylated chemical structure | VEGFR2/KDR Protein, Human, Recombinant (aa 20-764, His & Avi), Biotinylated molecular weight