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Anti-Whitlow linker Antibody (1M598) is a Mouse antibody targeting Whitlow linker. Anti-Whitlow linker Antibody (1M598) can be used in ELISA, WB, FCM.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $328 | 7-10 days | |
100 μL | $597 | 7-10 days |
Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, FCM Reactivity: Human |
Alias | Whitlow/218 Linker (E3U7Q) |
Ig Type | IgG2b |
Clone | 1M598 |
Reactivity | Human |
Verified Activity | 1. The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC50 is 5.147 to 5.761 ng/mL. 2. Western Blot -Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate -All lanes: Whitlow linker antibody at 1:1000 -Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution -Predicted band size: 50-200 kDa -Observed band size: 50-200 KDa -Exposure time:5s 3. Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry. |
Application | ELISA, WB, FCM |
Recommended Dose | WB:1:1000-1:5000; FCM:1:50-1:200. |
Antibody Type | Monoclonal |
Host Species | Mouse |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Purity | >95% |
Conjucates | Unconjugated |
Others Formats | FITC |
Antibody Types Available | 2 |
Immunogen | Whitlow linker-KLH |
Biology Area | Others |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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