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Anti-Whitlow linker Antibody (1M598)

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Catalog No. TMAH-01251
Alias Whitlow/218 Linker (E3U7Q)

Anti-Whitlow linker Antibody (1M598) is a Mouse antibody targeting Whitlow linker. Anti-Whitlow linker Antibody (1M598) can be used in ELISA, WB, FCM.

Anti-Whitlow linker Antibody (1M598)

Anti-Whitlow linker Antibody (1M598)

😃Good
Catalog No. TMAH-01251Alias Whitlow/218 Linker (E3U7Q)
Anti-Whitlow linker Antibody (1M598) is a Mouse antibody targeting Whitlow linker. Anti-Whitlow linker Antibody (1M598) can be used in ELISA, WB, FCM.
Pack SizePriceAvailabilityQuantity
50 μL$328 7-10 days
100 μL$597 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Mouse Monoclonal

Application: ELISA, WB, FCM

Reactivity: Human
AliasWhitlow/218 Linker (E3U7Q)
Ig Type
IgG2b
Clone
1M598
Reactivity
Human
Verified Activity
1. The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC50 is 5.147 to 5.761 ng/mL.
2. Western Blot
-Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate
-All lanes: Whitlow linker antibody at 1:1000
-Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution
-Predicted band size: 50-200 kDa
-Observed band size: 50-200 KDa
-Exposure time:5s
3. Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*10^6 cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
Application
ELISA, WB, FCM
Recommended Dose
WB:1:1000-1:5000; FCM:1:50-1:200.
Antibody Type
Monoclonal
Host SpeciesMouse
ConstructionHybridoma Monoclonal Antibody
PurificationProtein G purified
AppearanceLiquid
FormulationPreservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4.
Purity>95%
Related Conjugates and Formulations
Conjucates
Unconjugated
Others Formats
FITC
Antibody Types Available
2
Antigen Details
Immunogen
Whitlow linker-KLH
Biology Area
Others
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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