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Anti-STAG2 Antibody (9P946)
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Catalog No. TMAH-01135
Alias Stromal antigen 2, STAG 2, SCC3 homolog 2, SA2, Cohesin subunit SA-2
Anti-STAG2 Antibody (9P946) is an antibody targeting STAG2. Anti-STAG2 Antibody (9P946) can be used in ELISA, WB, IF.
Anti-STAG2 Antibody (9P946)
Anti-STAG2 Antibody (9P946)
😃Good
Catalog No. TMAH-01135Alias Stromal antigen 2, STAG 2, SCC3 homolog 2, SA2, Cohesin subunit SA-2
Anti-STAG2 Antibody (9P946) is an antibody targeting STAG2. Anti-STAG2 Antibody (9P946) can be used in ELISA, WB, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $209 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IF Reactivity: Human |
| Alias | Stromal antigen 2, STAG 2, SCC3 homolog 2, SA2, Cohesin subunit SA-2 |
| Ig Type | Rabbit IgG |
| Clone | 9P946 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate -All lanes: SA2 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 142, 146 kDa -Observed band size: 142 kDa 2. Immunofluorescence staining of MCF7 Cells with TMAH-01135 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | ELISA, WB, IF |
| Recommended Dose | WB:1:500-1:5000; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human SA2 |
| Antigen Species | Human |
| Gene ID | 10735 |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cell biology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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