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Anti-Argonaute-2 Antibody (6B282)
🥰Excellent
Catalog No. TMAY-03398
Anti-Argonaute-2 Antibody (6B282) is a Rabbit antibody targeting Argonaute-2. Anti-Argonaute-2 Antibody (6B282) can be used in ELISA,IHC-P,IP.
Anti-Argonaute-2 Antibody (6B282)
Anti-Argonaute-2 Antibody (6B282)
🥰Excellent
Catalog No. TMAY-03398
Anti-Argonaute-2 Antibody (6B282) is a Rabbit antibody targeting Argonaute-2. Anti-Argonaute-2 Antibody (6B282) can be used in ELISA,IHC-P,IP.
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Product Introduction
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Chemical Properties
| Description | Anti-Argonaute-2 Antibody (6B282) is a Rabbit antibody targeting Argonaute-2. Anti-Argonaute-2 Antibody (6B282) can be used in ELISA,IHC-P,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6B282 |
| Reactivity | Mouse |
| Verified Activity | 1. Immunochemical staining of AGO2 in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 2. AGO2 was immunoprecipitated using: -Lane A:0.5 mg K562 Whole Cell Lysate. -Lane B:0.5 mg Raw264.7 Whole Cell Lysate. -2 µL anti-Mouse AGO2 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-Mouse AGO2 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 100 kDa. -Observed band size: 100 kDa. 3. Immunochemical staining of AGO2 in human brain with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections. 4. AGO2 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg MCF-7 Whole Cell Lysate. -Lane C:0.5 mg HEK293 Whole Cell Lysate. -4 µL anti-AGO2 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. -Primary antibody: -Anti-AGO2 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size: 100 kDa. -Observed band size:100 kDa |
| Application | ELISA,IHC-P,IP |
| Recommended Dose | ELISA: 1:5000-1:10000; IHC-P: 1:100-1:500; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse AGO2 (rM AGO2; TMPY-02130; Q8CJG0; Met1-Ala860). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Mouse AGO2 protein (TMPY-02130) |
| Antigen Species | Mouse |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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