Anti-CD40 Antibody (4P686) is an antibody targeting CD40. Anti-CD40 Antibody (4P686) can be used in ELISA, IF, FC.

Mouse IgG2a

4P686

Human

1. Immunofluorescence staining of A549 cell with TMAH-00197 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).
2. Immunofluorescence staining of PC-3 cell with TMAH-00197 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).
3. Overlay Peak curve showing THP-1 cells surface stained with TMAH-00197 (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,012 events was performed.

ELISA, IF, FC

IF:1:20-1:200; FC:1:20-1:200.

Monoclonal

Unconjugated

Recombinant Protein: Human CD40 Protein

Human

958

Cardiovascular, Immunology, Stem cells