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Anti-ETHE1 Antibody (6K797)
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Catalog No. TMAY-02767
Alias YF13H12, HSCO, ethylmalonic encephalopathy 1
Anti-ETHE1 Antibody (6K797) is a Rabbit antibody targeting ETHE1. Anti-ETHE1 Antibody (6K797) can be used in WB,ELISA,IP.
Anti-ETHE1 Antibody (6K797)
Anti-ETHE1 Antibody (6K797)
😃Good
Catalog No. TMAY-02767Alias YF13H12, HSCO, ethylmalonic encephalopathy 1
Anti-ETHE1 Antibody (6K797) is a Rabbit antibody targeting ETHE1. Anti-ETHE1 Antibody (6K797) can be used in WB,ELISA,IP.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
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Antigen Details
Chemical Properties
| Description | Anti-ETHE1 Antibody (6K797) is a Rabbit antibody targeting ETHE1. Anti-ETHE1 Antibody (6K797) can be used in WB,ELISA,IP. |
| Alias | YF13H12, HSCO, ethylmalonic encephalopathy 1 |
| Ig Type | Monoclonal Rabbit IgG |
| Clone | 6K797 |
| Reactivity | Human |
| Verified Activity | 1. Anti-ETHE1 rabbit monoclonal antibody at 1:500 dilution. -Lane A: HepG2 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:28 kDa. -Observed band size:28 kDa. 2. ETHE1 was immunoprecipitated using: -Lane A:0.5 mg Hela Whole Cell Lysate. -Lane B:0.5 mg Jurkat Whole Cell Lysate. -2 µL anti-ETHE1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-ETHE1 rabbit monoclonal antibody, at 1:100 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 28 kDa. -Observed band size: 28 kDa |
| Application | WB,ELISA,IP |
| Recommended Dose | WB: 1:500-1:2000; ELISA: 1:5000-1:10000; IP: 1-4 μL/mg of lysate |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ETHE1 / HSCO (rh ETHE1 / HSCO; TMPY-03658; O95571; Leu13-Ala254). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | ETHE1, also known as HSCO, is a sulfur dioxygenase that localizes within the mitochondrial matrix. ETHE1 probably plays an important role in metabolic homeostasis in mitochondria. It may also function as a nuclear-cytoplasmic shuttling protein that binds transcription factor RELA/NFKB3 in the nucleus and exports it to the cytoplasm. ETHE1 can suppresses p53-induced apoptosis by preventing nuclear localization of RELA. Mutations in ETHE1 gene result in ethylmalonic encephalopathy. Ethylmalonic encephalopathy is an autosomal recessive, invariably fatal disorder characterized by early-onset encephalopathy, microangiopathy, chronic diarrhea, defective cytochrome c oxidase (COX) in muscle and brain, high concentrations of C4 and C5 acylcarnitines in blood and high excretion of ethylmalonic acid in urine. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Human ETHE1 / HSCO Protein (TMPY-03658) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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