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Anti-CD14 Antibody (7C57)
😃Good
Catalog No. TMAH-00167
Alias monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen
Anti-CD14 Antibody (7C57) is a Mouse antibody targeting CD14. Anti-CD14 Antibody (7C57) can be used in ELISA, WB, IHC, IF.
Anti-CD14 Antibody (7C57)
Anti-CD14 Antibody (7C57)
😃Good
Catalog No. TMAH-00167Alias monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen
Anti-CD14 Antibody (7C57) is a Mouse antibody targeting CD14. Anti-CD14 Antibody (7C57) can be used in ELISA, WB, IHC, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: ELISA, WB, IHC, IF Reactivity: Human, Mouse, Rat, Rabbit |
| Alias | monocyte differentiation antigen CD14, CD14 molecule, CD14 antigen |
| Ig Type | IgG1 |
| Clone | 7C57 |
| Reactivity | Human, Mouse, Rat, Rabbit |
| Verified Activity | 1. Western Blot -Positive WB detected in: Rabbit lung tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit small intestine tissue -All lanes: CD14 antibody at 1:2500 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 2. Western Blot -Positive WB detected in: Mouse kidney tissue, Rat liver tissue -All lanes: CD14 antibody at 1:2000 SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 3. Western Blot -Positive WB detected in: NIH/3T3 whole cell lysate -All lanes: CD14 antibody at at 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 4. Western Blot -Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate -All lanes: CD14 antibody at 1:1000 -Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilution -Predicted band size: 41 kDa -Observed band size: 55 kDa 5. Immunofluorescence staining of A549 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 6. Immunofluorescence staining of HepG2 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). 7. Immunofluorescence staining of Raw264.7 cells with TMAH-00167 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). |
| Application | ELISA, WB, IHC, IF |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| Purity | >95% |
| Research Background | Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-). |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human Monocyte differentiation antigen CD14 Protein (20-345AA) |
| Antigen Species | Human |
| Gene ID | 929 |
| Uniprot ID | |
| Biology Area | Immunology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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