Immobilized Human IL-23R, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 0.87 μg/ml determined by ELISA.

IL-23 Protein, Human, Recombinant (His & Avi), Biotinylated V2 is expressed in HEK293 Cells. The accession number is Q9NPF7(IL-23 alpha)&P29460(IL-12 beta).

Human

HEK293 Cells

SGRF,P19,IL23P19,IL-23A,IL-23,IL23

Arg20-Pro189(IL-23 alpha) & Ile23-Ser328(IL-12 beta)

> 95% as determined by Tris-Bis PAGE

Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The interleukin (IL)-12 family of cytokines, including IL12 and IL 23, play an important role in driving aberrant Th1 and Th17 immune responses in patients with Crohn's disease (CD). Targeting this pathway has opened new avenues for therapeutic intervention. The balance of proinflammatory cytokines interleukin (IL)-12 and IL-23 plays a key role in shaping the development of antitumor or protumor immunity.