Frizzled 5 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-05489

Frizzled 5 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.7 kDa and the accession number is Q13467.

Frizzled 5 Protein, Human, Recombinant (hFc)

Catalog No. TMPY-05489

Frizzled 5 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.7 kDa and the accession number is Q13467.

Pack Size	Price	Availability	Quantity
50 μg	$357	7-10 days
500 μg	$2,330	7-10 days

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Product Information

Biological Activity	Immobilized Recombinant Human Frizzled 5 / FZD5 Protein (Fc Tag) at 2μg/mL (100μL/well) can bind pan-FZD antibody, Human IgG2 , the EC50 is 19-55 ng/mL.
Description	Frizzled 5 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 42.7 kDa and the accession number is Q13467.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q13467
Synonyms	HFZ5,frizzled class receptor 5,C2orf31
Construction	A DNA sequence encoding the human FZD5 (NP_003459.2) (Met1-Pro167) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Ala 27
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	42.7 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Wnt signaling is involved in a variety of embryonic development processes of nonvertebrates and vertebrates, where it determines cell motility, cell polarity, differentiation, proliferation and apoptosis, as well as formation of neural synapses. Various homologs of the Wingless protein, termed WNT factors, represent key mediators and act through a receptor complex comprised of members of the Frizzled and low density lipoprotein-related receptors (LRP). 19 WNTs, 1 Frizzled, and 2 LRP proteins have been identified. Frizzled is a family of G protein-coupled receptor proteins consisting of a divergent signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail.Frizzled 5 (FZD5) is believed to be the receptor for the Wnt5A ligand, and also interactions with Wnt1B, Wnt2B, and Wnt 7A functionally. Recent studies of WNT5A/Frizzled-5 signaling have revealed an unexpected and novel role in orchestrating adaptive immunity in response to microbial stimulation. In addition, FZD5 is also implicated in the survival of mature neurons in the parafascicular nucleus of the thalamus.