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Anti-CD55/DAF Antibody (6D933)
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Catalog No. TMAH-00210
Alias TC, DAF, CROM, CR, CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Anti-CD55/DAF Antibody (6D933) is an antibody targeting CD55/DAF. Anti-CD55/DAF Antibody (6D933) can be used in ELISA, WB, IHC.
Anti-CD55/DAF Antibody (6D933)
Anti-CD55/DAF Antibody (6D933)
😃Good
Catalog No. TMAH-00210Alias TC, DAF, CROM, CR, CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Anti-CD55/DAF Antibody (6D933) is an antibody targeting CD55/DAF. Anti-CD55/DAF Antibody (6D933) can be used in ELISA, WB, IHC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $208 | 7-10 days | |
| 100 μL | $349 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC Reactivity: Human |
| Alias | TC, DAF, CROM, CR, CD55 molecule, decay accelerating factor for complement (Cromer blood group) |
| Ig Type | Rabbit IgG |
| Clone | 6D933 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate -All lanes: CD55 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 42, 49, 40, 57, 60 kDa -Observed band size: 70 kDa 2. IHC image of TMAH-00210 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-00210 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. |
| Application | ELISA, WB, IHC |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 3]: Secreted.; [Isoform 4]: Secreted.; [Isoform 5]: Secreted.; [Isoform 6]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 7]: Cell membrane; Lipid-anchor, GPI-anchor. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage. (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5. (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable). (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human CD55 |
| Antigen Species | Human |
| Gene ID | 1604 |
| Uniprot ID | |
| Biology Area | Immunology |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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