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Anti-IRAK4 Antibody (3T950)
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Catalog No. TMAH-00637
Alias REN64, NY-REN-64, IRAK-4, IPD1, interleukin-1 receptor-associated kinase 4
Anti-IRAK4 Antibody (3T950) is an antibody targeting IRAK4. Anti-IRAK4 Antibody (3T950) can be used in ELISA, WB, FC.
Anti-IRAK4 Antibody (3T950)
Anti-IRAK4 Antibody (3T950)
😃Good
Catalog No. TMAH-00637Alias REN64, NY-REN-64, IRAK-4, IPD1, interleukin-1 receptor-associated kinase 4
Anti-IRAK4 Antibody (3T950) is an antibody targeting IRAK4. Anti-IRAK4 Antibody (3T950) can be used in ELISA, WB, FC.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $207 | 7-10 days | |
| 100 μL | $348 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Recombinant Monoclonal Application: ELISA, WB, FC Reactivity: Human |
| Alias | REN64, NY-REN-64, IRAK-4, IPD1, interleukin-1 receptor-associated kinase 4 |
| Ig Type | Rabbit IgG |
| Clone | 3T950 |
| Reactivity | Human |
| Verified Activity | 1. Western Blot -Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate -All lanes: IRAK4 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 52, 38 kDa -Observed band size: 55 kDa 2. Overlay histogram showing Jurkat cells stained with TMAH-00637 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1µg/1*10^6 cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
| Application | ELISA, WB, FC |
| Recommended Dose | WB:1:500-1:5000; FC:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Cytoplasm. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human IRAK4 |
| Antigen Species | Human |
| Gene ID | 51135 |
| Uniprot ID | |
| Biology Area | Cardiovascular, Immunology, Signal transduction |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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