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E-Selectin/CD62E Protein, Human, Recombinant (His & Avi)
(Synonyms: selectin E, SELE, RP1-117P20.2, LECAM2, E-Selectin, ESEL, ELAM1, ELAM, CD62E) Copy Product InfoSynonyms: selectin E, SELE, RP1-117P20.2, LECAM2, E-Selectin, ESEL, ELAM1, ELAM, CD62E
Catalog No. TMPK-00349 Copy Product Info
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis. E-Selectin/CD62E Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 61.6 kDa and the accession number is P16581.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $32 | 7-10 days | 7-10 days | |
| 10 μg | $48 | 7-10 days | 7-10 days | |
| 20 μg | $74 | 7-10 days | 7-10 days | |
| 50 μg | $138 | 7-10 days | 7-10 days | |
| 100 μg | $231 | 7-10 days | 7-10 days | |
| 200 μg | $419 | 7-10 days | 7-10 days | |
| 500 μg | $932 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Human E-Selectin, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA. |
| Description | E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis. E-Selectin/CD62E Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 61.6 kDa and the accession number is P16581. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P16581 |
| Construction | Trp22-Pro556 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | selectin E, SELE, RP1-117P20.2, LECAM2, E-Selectin, ESEL, ELAM1, ELAM, CD62E |
| Research Background |
| Molecular Weight | 61.6 kDa (predicted). Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: E-Selectin/CD62E Protein, Human, Recombinant (His & Avi) chemical structure | E-Selectin/CD62E Protein, Human, Recombinant (His & Avi) molecular weight
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