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CD40 Ligand Protein, Human, Recombinant (His)

Catalog No. TMPY-01758
CD40 Ligand Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 17.7 kDa and the accession number is P29965.
Pack SizePriceAvailabilityQuantity
100 μg$4987-10 days
1 mg$3,5207-10 days
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Product Information

Biological Activity
Immobilized Human CD40 Ligand (His Tag) at 2 μg/mL (100 μL/well) can bind Human CD40 (ECD, hFc Tag), the EC50 of Human CD40 (ECD, hFc Tag) is 60-600 ng/mL.
Description
CD40 Ligand Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 17.7 kDa and the accession number is P29965.
Species
Human
Expression System
E. coli
TagC-His
Accession NumberP29965
Synonyms
TRAP,TNFSF5,T-BAM,IMD3,IGM,HIGM1,hCD40L,gp39,CD40L,CD40 Ligand,CD154
Construction
A DNA sequence encoding the soluble form of human CD40L (NP_000065.1) (Glu 108-Leu 261) was expressed with an initial Met at the N-terminus and a polyhistide tag at the C-terminus. Predicted N terminal: Met
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.
Molecular Weight17.7 kDa (predicted); 18 kDa (reducing conditions)
EndotoxinPlease contact us for more information.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

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