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Anti-OTUB2 Antibody (4V813)
😃Good
Catalog No. TMAY-02522
Alias OTU2, OTU deubiquitinase, ubiquitin aldehyde binding 2, OTB2, C14orf137
Anti-OTUB2 Antibody (4V813) is a Rabbit antibody targeting OTUB2. Anti-OTUB2 Antibody (4V813) can be used in ELISA,ICC/IF.
Anti-OTUB2 Antibody (4V813)
Anti-OTUB2 Antibody (4V813)
😃Good
Catalog No. TMAY-02522Alias OTU2, OTU deubiquitinase, ubiquitin aldehyde binding 2, OTB2, C14orf137
Anti-OTUB2 Antibody (4V813) is a Rabbit antibody targeting OTUB2. Anti-OTUB2 Antibody (4V813) can be used in ELISA,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μL | $269 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, ICC/IF Reactivity: Human |
| Alias | OTU2, OTU deubiquitinase, ubiquitin aldehyde binding 2, OTB2, C14orf137 |
| Ig Type | Rabbit IgG |
| Clone | 4V813 |
| Reactivity | Human |
| Verified Activity | Immunofluorescence staining of Human OTUB2 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human OTUB2 monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 549-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus. |
| Application | ELISA,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human OTUB2 (rh OTUB2; TMPY-02522; Q96DC9-1; Met1-His234). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Otubain 2 (OTUB2) is a member of DUBs that belong to the ovarian tumour (OTU) superfamily of proteins which consists of a five-stranded β-sheet sandwiched in between a small helical amino-terminal region consisting of α1 and α2, and a large helical region comprised of α3-α10. Like other DUBs, otubain 2 (OTUB2) cleaves proteins precisely at the ubiquitin-protein bond so that ubiquitylation process can be reversed and regulated. Otubain 2 (OTUB2)'s active-site cleft is sterically occluded by a novel loop conformation resulting in an oxyanion hole, which consists uniquely of backbone amides. Furthermore, the residues that orient and stabilize the active-site histidine of otubain 2 (OTUB2) are different from other cysteine proteases. This reorganization of the active-site topology provides a possible explanation for the low turnover and substrate specificity of the otubains. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Human OTUB2 Protein (TMPY-02522) |
| Antigen Species | Human |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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