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Anti-EpCAM Antibody (4H719) is an antibody targeting EpCAM. Anti-EpCAM Antibody (4H719) can be used in ELISA,FCM,IHC.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $347 | 7-10 days |
Description | Anti-EpCAM Antibody (4H719) is an antibody targeting EpCAM. Anti-EpCAM Antibody (4H719) can be used in ELISA,FCM,IHC. |
Synonyms | TROP-1, TROP1, TACSTD1, MK-1, MIC18, M4S1, KSA, KS1/4, HNPCC8, ESA, epithelial cell adhesion molecule, EGP40, EGP314, EGP-2, DIAR5 |
Ig Type | Mouse IgG2a |
Clone | 4H719 |
Reactivity | Human |
Verified Activity | 1. IHC image of TMAH-00398 diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. 2. Overlay Peak curve showing MCF7 cells surface stained with TMAH-00398 (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed. |
Application | |
Recommended Dose | IHC:1:20-1:200; FCM:1:20-1:200. |
Antibody Type | Monoclonal |
Subcellular Localization | Lateral cell membrane; Single-pass type I membrane protein. Cell junction, tight junction. |
Tissue Specificity | Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
Research Background | May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. |
Conjucates | Unconjugated |
Immunogen | Recombinant Protein: Human EPCAM Protein |
Antigen Species | Human |
Gene Name | EPCAM |
Gene ID | |
Uniprot ID | |
Biology Area | Tags & Cell Markers |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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