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Anti-Calreticulin Polyclonal Antibody
😃Good
Catalog No. TMAB-00273
Alias SSA, RO, HEL-S-99n, CRT, cC1qR, calreticulin
Anti-Calreticulin Polyclonal Antibody is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Anti-Calreticulin Polyclonal Antibody
Anti-Calreticulin Polyclonal Antibody
😃Good
Catalog No. TMAB-00273Alias SSA, RO, HEL-S-99n, CRT, cC1qR, calreticulin
Anti-Calreticulin Polyclonal Antibody is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-Calreticulin Polyclonal Antibody is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA. |
| Alias | SSA, RO, HEL-S-99n, CRT, cC1qR, calreticulin |
| Ig Type | IgG |
| Reactivity | Human |
| Verified Activity | 1. Sample: Lane 1: Human Jurkat cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human MCF-7 cell lysates Primary: Anti-Calreticulin (TMAB-00273) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kDa Observed band size: 52 kDa 2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020] |
| Immunogen | KLH conjugated synthetic peptide: human Calreticulin |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | ER |
| Molecular Weight | Theoretical: 44 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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