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Anti-Calreticulin Polyclonal Antibody

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Catalog No. TMAB-00273

Anti-Calreticulin Polyclonal Antibody is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

Anti-Calreticulin Polyclonal Antibody

Anti-Calreticulin Polyclonal Antibody

😃Good
Catalog No. TMAB-00273
Anti-Calreticulin Polyclonal Antibody is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Pack SizePriceAvailabilityQuantity
50 μL$220 7-10 days
100 μL$372 7-10 days
200 μL$528 7-10 days
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Product Introduction

Bioactivity
Description
Anti-Calreticulin Polyclonal Antibody is a Rabbit antibody targeting Calreticulin. Anti-Calreticulin Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.
Ig Type
IgG
Reactivity
Human
Verified Activity
1. Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human HepG2 cell lysates
Lane 3: Human MCF-7 cell lysates
Primary: Anti-Calreticulin (TMAB-00273) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 52 kDa
2. Paraformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (TMAB-00273) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Application
WB,IHC-P,IHC-Fr,IF,ELISA
Recommended Dose
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationEndoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundCalreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020]
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human Calreticulin
Antigen Species
Human
Gene ID
Uniprot ID
Biology Area
ER
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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