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CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated

(Synonyms: IL2RAC, Il2ra) Copy Product Info

Synonyms: IL2RAC, Il2ra

Catalog No. TMPY-07247 Copy Product Info
CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is P26897.
CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated
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Pack SizePriceUSA StockGlobal StockQuantity
5 μg$2087-10 days7-10 days
10 μg$3437-10 days7-10 days
20 μg$5887-10 days7-10 days
50 μg$9397-10 days7-10 days
100 μg$1,6007-10 days7-10 days
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction

Bioactivity
Bioactivity
Immobilized Recombinant Rat CD25 / IL-2R alpha Protein (ECD, His & AVI Tag), Biotinylated at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human IL2 Protein, Low Endotoxin, the EC50 is 5-15 ng/mL.
Description
CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated is expressed in Baculovirus-Insect Cells. The accession number is P26897.
Species
Rat
Expression System
Baculovirus Insect Cells
TagC-His-Avi
Accession NumberP26897
ConstructionA DNA sequence encoding the Rat IL2RA (NP_037295.1) (Met1-Gln235) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Protein Purity
≥ 95% as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from sterile 20 mM Tris, 300 mM NaCl, 10% Glycerol, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
ReconstitutionPlease refer to the lot-specific COA.
Stability & StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
SynonymsIL2RAC, Il2ra
Research Background
Chemical Properties
Molecular Weight27.72 kDa (predicted); 41.2 kDa (reducing contition, due to glycosylation)
Storage & Solubility Information
StorageLyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months

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Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Related Tags: CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated chemical structure | CD25/IL2R alpha Protein, Rat, Recombinant (His & Avi), Biotinylated molecular weight