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ANGPT2/Angiopoietin-2 Protein, Cynomolgus, Rhesus, Recombinant (hFc)

Catalog No. TMPY-04188
ANGPT2/Angiopoietin-2 Protein, Cynomolgus, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 53.8 kDa and the accession number is XP_005562586.1&XP_001097949.1.
Pack SizePriceAvailabilityQuantity
100 μg$6187-10 days
1 mg$3,0007-10 days
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Product Information

Biological Activity
Measured by its binding ability in a functional ELISA. Immobilized Rhesus TIE2 His at 2 μg/ml (100 μl/well) can bind Cynomolgus, Rhesus Angiopoietin-2hFc , the EC50 of Cynomolgus, Rhesus Angiopoietin-2hFc is 6.0-48.0 ng/mL.
Description
ANGPT2/Angiopoietin-2 Protein, Cynomolgus, Rhesus, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 53.8 kDa and the accession number is XP_005562586.1&XP_001097949.1.
Species
Cynomolgus,Rhesus
Expression System
HEK293 Cells
TagN-hFc
Accession NumberXP_005562586.1&XP_001097949.1
Synonyms
angiopoietin 2
Construction
A DNA sequence encoding the cynomolgus / rhesus ANGPT2 (XP_005562586.1/XP_001097949.1) (Lys274-Phe495) was expressed with the Fc region of human IgG1 at the N-terminus. Cynomolgus and Rhesus ANGPT2 sequences are identical. Predicted N terminal: Glu
Protein Purity
> 95 % as determined by SDS-PAGE.
Molecular Weight53.8 kDa (predicted)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.

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