Anti-ADAMTS13 Polyclonal Antibody is a Rabbit antibody targeting ADAMTS13. Anti-ADAMTS13 Polyclonal Antibody can be used in ELISA, FCM, ICC/IF, WB.

IgG

Human

1. 25 ug total protein per lane of various lysates (see on figure) probed with ADAMTS13 polyclonal antibody, unconjugated (TMAB-02301) at 1: 1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r. T. for 60 min.
2. The A549 (H) cells were fixed with 4% PFA (10 min at r. T.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r. T.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-ADAMTS13 antibody (TMAB-02301): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG. Blank control (black): PBS. Acquisition of 20,000 events was performed.
3. 4% Paraformaldehyde-fixed A549 (H) cell; Triton X-100 at r. T. for 20 min; Antibody incubation with (ADAMTS13) polyclonal Antibody, unconjugated (TMAB-02301) 1: 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, BF488) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

ELISA=1:5000-10000; FCM=1 μg/Test; ICC/IF=1:50-200; WB=1:500-2000

Polyclonal

Recombinant Protein: human ADAMTS13 protein

Human

ADAMTS13

A disintegrin and metalloproteinase with thrombospondin motifs 13

Cleaves the vWF multimers in plasma into smaller forms.