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Anti-Her2/ERBB2 Antibody (6Z596)
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Catalog No. TMAY-00042
Alias erb-b2 receptor tyrosine kinase 2, EGFR2
Anti-Her2/ERBB2 Antibody (6Z596) is a Rabbit antibody targeting Her2/ERBB2. Anti-Her2/ERBB2 Antibody (6Z596) can be used in ELISA,ELISA(Cap),FCM,ICC/IF.
Anti-Her2/ERBB2 Antibody (6Z596)
Anti-Her2/ERBB2 Antibody (6Z596)
😃Good
Catalog No. TMAY-00042Alias erb-b2 receptor tyrosine kinase 2, EGFR2
Anti-Her2/ERBB2 Antibody (6Z596) is a Rabbit antibody targeting Her2/ERBB2. Anti-Her2/ERBB2 Antibody (6Z596) can be used in ELISA,ELISA(Cap),FCM,ICC/IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Monoclonal Application: ELISA, ELISA(Cap), FCM, ICC/IF Reactivity: Human |
| Alias | erb-b2 receptor tyrosine kinase 2, EGFR2 |
| Ig Type | Rabbit IgG |
| Clone | 6Z596 |
| Reactivity | Human |
| Verified Activity | 1. Immunofluorescence staining of Human ErbB2 in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human ErbB2 monoclonal antibody (1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Flow cytometric analysis of anti-ErbB2 on SK-BR3 cells. SK-BR3 cells were detached using 1X trypsin, washed, then stained with Purified Rabbit anti-Human ErbB2. Second step staining with FITC conjugated Goat anti-Rabbit IgG polyclonal antibody (BD Pharmingen™, Cat.No 554020). 3. Flow cytometric analysis of anti-ErbB2 on MCF-7 cells. MCF-7 cells were detached using 1X trypsin, washed, then stained with purified rabbit anti-human ErbB2. Second step staining with FITC conjugated goat anti-rabbit IgG (H+L) polyclonal antibody. |
| Application | ELISA,ELISA(Cap),FCM,ICC/IF |
| Recommended Dose | ELISA: 1:5000-1:10000; ICC-IF: 1:50-1:1000; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Construction | This antibody was obtained from a rabbit immunized with purified, recombinant Human ErbB2 / HER2 extracellular domain (rh ErbB2; TMPY-01137; NP_004439.2; Met 1-Thr 652). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| Conjucates | Unconjugated |
| Others Formats | APC/Biotin/FITC/PE |
| Antibody Types Available | 5 |
| Immunogen | Recombinant Protein: Human ErbB2 protein (TMPY-01137) |
| Antigen Species | Human |
| Biology Area | Cancer Drug Targets, Receptor Tyrosine Kinases (RTKs) |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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