SIRP alpha V8 Protein, Human, Recombinant (mFc)

Name: SIRP alpha V8 Protein, Human, Recombinant (mFc)
Brand: TargetMol
SKU: TMPY-06055
Rating: 4.5 (1 reviews)

(Synonyms: SIRP α(SIRP αV8), SIRP α, SIRP alpha, SIRP, signal-regulatory protein α, signal-regulatory protein alpha, SHPS1, PTPNS1, P84, MYD-1, MFR, CD172A, BIT) Copy Product Info

Synonyms: SIRP α(SIRP αV8), SIRP α, SIRP alpha, SIRP, signal-regulatory protein α, signal-regulatory protein alpha, SHPS1, PTPNS1, P84, MYD-1, MFR, CD172A, BIT

Catalog No. TMPY-06055 Copy Product Info

SIRP alpha V8 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 63.6 kDa and the accession number is AAH38510.1.

Pack Size	Price	USA Stock	Global Stock
5 µg	$79	7-10 days	7-10 days
10 µg	$129	7-10 days	7-10 days
20 µg	$208	7-10 days	7-10 days
50 µg	$419	7-10 days	7-10 days
100 µg	$812	7-10 days	7-10 days

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Information

Bioactivity

Bioactivity	Measured by its binding ability in a functional ELISA. Immobilized human CD47-His at 2 μg/ml (100 μl/well) can bind human SIRPa(V8)-mFc , the EC50 of human SIRPa(V8)-mFc is 2-14 ng/mL.
Description	SIRP alpha V8 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 63.6 kDa and the accession number is AAH38510.1.
Species	Human
Expression System	HEK293 Cells
Tag	C-mFc
Accession Number	AAH38510.1
Construction	A DNA sequence encoding the human SIRP alphaV8 (AAH38510.1, with mutation Ser 44 Leu, Ser 50 Thr, Ile 52 Thr, His 54 Arg, Val 57 Ala) (Met1-Arg369) was expressed with the Fc region of mouse IgG2a at the C-terminus. Predicted N terminal: Glu 31
Protein Purity	> 95 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonyms	SIRP α(SIRP αV8), SIRP α, SIRP alpha, SIRP, signal-regulatory protein α, signal-regulatory protein alpha, SHPS1, PTPNS1, P84, MYD-1, MFR, CD172A, BIT
Research Background	Tyrosine-protein phosphatase non-receptor type substrate 1, also known as SHP substrate 1, Inhibitory receptor SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Macrophage fusion receptor, CD172 antigen-like family member A, SIRPA and CD172a, is a single-pass type I membrane protein which contains two Ig-like C1-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. SIRPA is ubiquitously expressed. It is highly expressed in brain and detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. It is also detected on myeloid cells, but not T-cells. SIRPA is an immunoglobulin-like cell surface receptor for CD47. SIRPA acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. It may play a key role in intracellular signaling during synaptogenesis and in synaptic function. SIRPA is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. It mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: IP AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

Chemical Properties

Molecular Weight

63.6 kDa (predicted); 80.4 kDa (reducing condition, due to glycosylation)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.