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Anti-CD80 Antibody (6N172)

😃Good
Catalog No. TMAH-00223
Alias LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7

Anti-CD80 Antibody (6N172) is an antibody targeting CD80. Anti-CD80 Antibody (6N172) can be used in ELISA, WB, IHC, IP.

Anti-CD80 Antibody (6N172)

Anti-CD80 Antibody (6N172)

😃Good
Catalog No. TMAH-00223Alias LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7
Anti-CD80 Antibody (6N172) is an antibody targeting CD80. Anti-CD80 Antibody (6N172) can be used in ELISA, WB, IHC, IP.
Pack SizePriceAvailabilityQuantity
50 μL$209 7-10 days
100 μL$349 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Recombinant Rabbit Monoclonal

Application: ELISA, WB, IHC, IP

Reactivity: Human
AliasLAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7
Ig Type
Rabbit IgG
Clone
6N172
Reactivity
Human
Verified Activity
1. Western Blot
-Positive WB detected in: Raji whole cell lysate
-All lanes: CD80 antibody at 1:2000
-Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution
-Predicted band size: 34, 30, 19 kDa
-Observed band size: 60 kDa
2. IHC image of TMAH-00223 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
3. Immunoprecipitating CD80 in Raji whole cell lysate
-Lane 1: Rabbit control IgG instead of TMAH-00223 in Raji whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
-Lane 2: TMAH-00223(2µg)+ Raji whole cell lysate(500µg)
-Lane 3: Raji whole cell lysate (10µg)
Application
ELISA, WB, IHC, IP
Recommended Dose
WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000.
Antibody Type
Monoclonal
Subcellular LocalizationMembrane; Single-pass type I membrane protein.
ConstructionRecombinant Antibody
PurificationAffinity-chromatography
AppearanceLiquid
FormulationPhosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Research BackgroundInvolved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
A synthetic peptide: Human CD80
Antigen Species
Human
Gene ID
941
Uniprot ID
Biology Area
Immunology, Stem cells
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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