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Anti-CD80 Antibody (6N172) is an antibody targeting CD80. Anti-CD80 Antibody (6N172) can be used in ELISA, WB, IHC, IP.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $209 | 7-10 days | |
100 μL | $349 | 7-10 days |
Description | Antibody Type: Recombinant Rabbit Monoclonal Application: ELISA, WB, IHC, IP Reactivity: Human |
Alias | LAB7, CD80 molecule, CD80, CD28LG1, BB1, B7-1, B71, B7.1, B7 |
Ig Type | Rabbit IgG |
Clone | 6N172 |
Reactivity | Human |
Verified Activity | 1. Western Blot -Positive WB detected in: Raji whole cell lysate -All lanes: CD80 antibody at 1:2000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 34, 30, 19 kDa -Observed band size: 60 kDa 2. IHC image of TMAH-00223 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. Immunoprecipitating CD80 in Raji whole cell lysate -Lane 1: Rabbit control IgG instead of TMAH-00223 in Raji whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) -Lane 2: TMAH-00223(2µg)+ Raji whole cell lysate(500µg) -Lane 3: Raji whole cell lysate (10µg) |
Application | ELISA, WB, IHC, IP |
Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IP:1:200-1:1000. |
Antibody Type | Monoclonal |
Subcellular Localization | Membrane; Single-pass type I membrane protein. |
Construction | Recombinant Antibody |
Purification | Affinity-chromatography |
Appearance | Liquid |
Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Research Background | Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B. |
Conjucates | Unconjugated |
Immunogen | A synthetic peptide: Human CD80 |
Antigen Species | Human |
Gene ID | 941 |
Uniprot ID | |
Biology Area | Immunology, Stem cells |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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