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Anti-RBBP4 Antibody (9I427) (Synonyms: retinoblastoma binding protein 4, RBAP48, mRbAp48)
Catalog No. TMAH-01034 Copy Product Info
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Anti-RBBP4 Antibody (9I427) is an antibody targeting RBBP4. Anti-RBBP4 Antibody (9I427) can be used in ELISA,IF,IHC,WB.
Anti-RBBP4 Antibody (9I427)
Copy Product Info🥰Excellent
Catalog No. TMAH-01034
Synonyms retinoblastoma binding protein 4, RBAP48, mRbAp48
Anti-RBBP4 Antibody (9I427) is an antibody targeting RBBP4. Anti-RBBP4 Antibody (9I427) can be used in ELISA,IF,IHC,WB.
Anti-RBBP4 Antibody
(9I427)
(9I427)
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 50 μL | $209 | 7-10 days | 7-10 days | |
| 100 μL | $348 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Related Conjugates and Formulations
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Chemical Properties
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| Description | Anti-RBBP4 Antibody (9I427) is an antibody targeting RBBP4. Anti-RBBP4 Antibody (9I427) can be used in ELISA,IF,IHC,WB. |
| Synonyms | retinoblastoma binding protein 4, RBAP48, mRbAp48 |
| Ig Type | Rabbit IgG |
| Clone | 9I427 |
| Reactivity | Human, Mouse, Rat |
| Verified Activity | 1. Western Blot -Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate -All lanes: RbAp48 antibody at 1:1000 -Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution -Predicted band size: 48, 48, 47, 44 kDa -Observed band size: 53, 40 kDa 2. IHC image of TMAH-01034 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 3. IHC image of TMAH-01034 diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. 4. Immunofluorescence staining of Hela Cells with TMAH-01034 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L). |
| Application | |
| Recommended Dose | WB:1:500-1:5000; IHC:1:50-1:200; IF:1:20-1:200. |
| Antibody Type | Monoclonal |
| Subcellular Localization | Nucleus. |
| Construction | Recombinant Antibody |
| Purification | Affinity-chromatography |
| Appearance | Liquid |
| Formulation | Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Research Background | Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex. |
| Conjucates | Unconjugated |
| Immunogen | A synthetic peptide: Human RbAp48 |
| Antigen Species | Human |
| Gene Name | RBBP4 |
| Gene ID | |
| Uniprot ID | |
| Biology Area | Epigenetics and Nuclear Signaling, Cancer |
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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