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Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated

(Synonyms: PVRL4, PRR4, nectin-4, LNIR, EDSS1) Copy Product Info

Synonyms: PVRL4, PRR4, nectin-4, LNIR, EDSS1

Catalog No. TMPY-06958 Copy Product Info
Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.

Batch Information

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Purity:≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Recombinant Human NECTIN4/Nectin 4 Protein (ECD, Fc & AVI Tag), Biotinylated on streptavidin precoated (2 μg/mL,100 μL/well) can bind Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag), the EC50 is 40-120 ng/mL.
Description
Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.
Species
Human
Expression System
HEK293 Cells
TagC-hFc-Avi
Accession NumberNP_112178.2
ConstructionA DNA sequence encoding the Human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly 32
Protein Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
SynonymsPVRL4, PRR4, nectin-4, LNIR, EDSS1
Chemical Properties
Molecular Weight62.65 kDa (predicted); 76.3 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated chemical structure | Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated molecular weight