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Anti-Flagellin Antibody (7U52)
😃Good
Catalog No. TMAY-02859
Alias flaA
Anti-Flagellin Antibody (7U52) is a Mouse antibody targeting Flagellin. Anti-Flagellin Antibody (7U52) can be used in WB,ELISA.
Anti-Flagellin Antibody (7U52)
Anti-Flagellin Antibody (7U52)
😃Good
Catalog No. TMAY-02859Alias flaA
Anti-Flagellin Antibody (7U52) is a Mouse antibody targeting Flagellin. Anti-Flagellin Antibody (7U52) can be used in WB,ELISA.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 20 μL | $92 | 7-10 days | |
| 100 μL | $268 | 7-10 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB, ELISA Reactivity: Listeria monocytogenes |
| Alias | flaA |
| Ig Type | Mouse IgG1 |
| Clone | 7U52 |
| Reactivity | Listeria monocytogenes |
| Verified Activity | 1. Anti-Listeria monocytogenes flagellin / flaA mouse monoclonal antibody at 1:1000 dilution. -Sample:Listeria monocytogenes flagellin / flaA Recombinant Protein -Lane A: 200ng -Lane B: 50ng -Secondary -Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. 2. Anti-Listeria monocytogenes flagellin / flaA mouse monoclonal antibody at 1:1000 dilution. -Sample:Listeria monocytogenes flagellin / flaA Recombinant Protein -Lane A: 10ng -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. |
| Application | WB,ELISA |
| Recommended Dose | WB: 1:1000-1:5000; ELISA: 1:1000-1:2000 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Construction | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Listeria monocytogenes Listeria monocytogenes flagellin / FlaA (TMPY-03465; YP_013330.1; Met1-Ser287). |
| Purification | Protein A |
| Appearance | Liquid |
| Formulation | 0.2 μm filtered solution in PBS |
| Research Background | The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a FlaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the FlaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases. |
| Conjucates | Unconjugated |
| Immunogen | Recombinant Protein: Listeria monocytogenes Listeria monocytogenes flagellin / FlaA protein (TMPY-03465) |
| Antigen Species | Listeria monocytogenes |
Stability & Storage
| Stability & Storage | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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